# NP-Sticky: Model-Guided Ligation Calculator

## Circular Ligation Calculator

You can download this Excel worksheet to facilitate concentration conversion and ligation reaction setup: ligation_worksheet.xlsx### Product Distribution Prediction

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This circular ligation calculator can predict product distribution given the input concentrations of vector and insert in the ligation reaction.
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**Please enter reaction conditions for insert-vector ligation reaction.**

### Ligation Recommendation

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Ligation incorporation, efficiency, and accuracy will be calculated over a range of concentrations and molar ratios to allow selection of optimal ligation conditions for specific applications.
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**Please enter reaction conditions for insert-vector ligation reaction.**

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

The Python source codes are available here: cirlig_predict.cgi; cirlig_recom.cgi

Last modified by Daphne Ng on Jan 14th, 2014